Journal: Cancer Medicine
Article Title: Clinical Significance and Therapeutic Potential of Long Non‐Coding RNA H19 in Soft Tissue Sarcoma
doi: 10.1002/cam4.71305
Figure Lengend Snippet: H19 expression in different sarcoma subtypes. (A) H19 RNA‐seq expression data across cell lines from 38 different cancer types derived from the publicly available CCLE database. (B) H19 RNA‐seq expression data from 31 cancer types in tumor tissue derived from TCGA and GTEx data (SARC = sarcoma; TMP = Transcript Per Million). (C) The expression of H19 in 7 different sarcoma cell lines was measured by qRT‐PCR and normalized to the housekeeper genes GAPDH and U6 ( n = 3; mean ± SD). (D) Representative pictures of RNA in situ hybridization of H19 in the liposarcoma cell line SW872 showing a heterogenous expression pattern.
Article Snippet: The sarcoma cell lines SW872 (Liposarcoma) and SW982 (Synovial Sarcoma) were purchased from the American Type Culture Collection (ATCC; Manassas, CA, USA).
Techniques: Expressing, RNA Sequencing, Derivative Assay, Quantitative RT-PCR, RNA In Situ Hybridization